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Two challenges often shorten the life span of an analytical column. To start with, solutes that bind irreversibly to the stationary stage degrade the column’s performance by decreasing the level of stationary phase accessible for effecting a separation. 2nd, particulate product injected with the sample might clog the analytical column.The sample
. After we take a look at the chromatograms from these 7 mobile phases we may perhaps discover that a number of presents an sufficient separation, or we might detect a region in the solvent triangle where a separation is possible.최상의 결과를 위해서는 올바른 시약을 사용함으로써 피크 대칭성을 개선할 수 있습니다.